中文名称:小鼠诱导型多能干细胞(iPS细胞)OSK-1,OSK-1,SHMCC E00286
英文名称:OSK-1
动物种类:小鼠,C57BL/6、Oct4-EGFP转基因品系
细胞描述:小鼠诱导型多能干细胞,每支细胞量1×10
6,通过重编程转录因子Oct4、Sox2和Klf4转染携带Oct4 promoter-driven EGFP的MEF从而诱导建立的iPS细胞。培养时需要饲养层细胞。饲养层为ICR MEF (
SCSP-107C 或
SCSP-107R )
性别:
组织来源:小鼠胚胎成纤维细胞
形态:球形克隆
培养基:小鼠iPS完全培养液(600ml):DMEM (Invitrogen 12430) 485 mlES级FBS (biochrom S4115) 90 mlGlutamax (Invitrogen 35050) 6 mlNEAA (Invitrogen 11140) 6 mlNucleosides (Millipore ES-008-D) 6 mlLIF (Millipore ESG1107) 60 μl(终浓度为1000U/ml)β-Mer (Invitrogen 21985) 1.5 mlPS (Millipore TMS-AB2-C) 6 ml气相:空气,95%;二氧化碳,5%。温度:37摄氏度。
参考文献:Jiang, J., Lv, W., Ye, X., Wang, L., Zhang, M., Yang, H., Okuka, M., Zhou, C., Zhang, X., Liu, L., et al. Zscan4 promotes genomic stability during eprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation. Cell research. 2013 Jan; 23(1):92-106 (封面文章).