中文名称：人肝癌细胞,Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B],SHMCC E00353
英文名称：Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B]
动物种类：人
细胞描述：该细胞系来自8岁黑人男性的组织。其染色体模式数目为60，在裸鼠中能致瘤。该细胞系含有乙型肝炎病毒基因组，需在2级生物安全防护下操作。每个批次均通过本库支原体检测，结果阴性。经本库STR检测结果无误。STR结果如下：Amelogenin: X；CSF1PO: 8；D13S317: 12,14；D16S539: 10；D5S818: 13；D7S820: 8,10；THO1: 6,7；TPOX: 9；vWA: 17。
性别：男性，8岁
组织来源：肝
形态：上皮样，贴壁生长
培养基：人肝癌细胞Hep 3B2.1-7 [Hep 3B, Hep-3B, Hep3B]完全培养液配方（100 ml）：MEM (Invitrogen，11090081) 87 mlFBS (Gibco) 10 mlGlutamax（invitrogen 35050061） 1 mlNon-essential Amino Acids, 100 (Invitrogen, 11140050) 1 mlSodium Pyruvate 100 mM Solution（invitrogen 11360070） 1 ml气相：空气，95%；二氧化碳，5%。温度：37摄氏度。参考传代比例：1:3参考传代周期：3-5天参考换液频率：每周2次冻存液配方：完全培养液95%，DMSO 5%
参考文献：Knowles BB, et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983Aden DP, et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137Darlington GJ, et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851Knowles BB, Aden DP. Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983
