中文名称：人大细胞肺癌细胞,NCI-H661 [H661],SHMCC E00361
英文名称：NCI-H661 [H661]
动物种类：白人
细胞描述：此细胞株源自一位45岁患有大细胞肺癌的白人男性的淋巴结。该细胞缺乏产生粘液和鳞状分化的亚显微结构和生化证据。其表达的p53 mRNA与正常肺细胞的表达量相近，且易于检测。此外，没有表现出明显的结构DNA异常。该细胞角蛋白和波形蛋白表达阳性，神经丝三联体蛋白阴性。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X,Y；CSF1PO: 10；D13S317: 11；D16S539: 11；D5S818: 12；D7S820: 8,12；TH01: 9.3；TPOX: 8；vWA: 17,18。
性别：男性，45岁
组织来源：肺；来源于转移部位：淋巴结
形态：上皮样，贴壁生长
培养基：人大细胞肺癌细胞NCI-H661 [H661] 完全培养液配方（100 ml）：RPMI 1640 Medium (Invitrogen, 11875-093) 88 mlFBS (Gibco) 10 mlGlutamax (Invitrogen, 35050061) 1 mlSodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml参考传代比例：1：3参考传代周期：3~5天参考换液频率：每周2次冻存液配方：完全培养液95%，DMSO 5%
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