中文名称：SV40转化的非洲绿猴肾细胞,COS-7,SHMCC E00315
英文名称：COS-7
动物种类：非洲绿猴
细胞描述：SV40转化的非洲绿猴肾细胞COS-7。此细胞株源自CV-1细胞株，经带有编码野生型T抗原的起始失活突变的SV40转染得到。每个批次均通过本库支原体检测，结果为阴性。
性别：
组织来源：肾脏
形态：成纤维细胞样，贴壁生长
培养基：COS-7 完全培养液 配方（100 ml）
DMEM (Invitrogen, 11960-044) 88 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
FBS (Gibco) 10 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。生物安全等级：BSL-2
参考传代周期：每周2-3次
参考传代比例：1：4~1：8
参考换液频率：每周2~3次
冻存液配方：完全培养液95%，DMSO 5%
参考文献：Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175-182, 1981. PubMed: 6260373 Fernandez LM, Puett D. Lys583 in the third extracellular loop of the lutropin/choriogonadotropin receptor is critical for signaling. J. Biol. Chem. 271: 925-930, 1996. PubMed: 8557706 Maestrini E, et al. A family of transmembrane proteins with homology to the MET-hepatocyte growth factor receptor. Proc. Natl. Acad. Sci. USA 93: 674-678, 1996. PubMed: 8570614 Campbell M, et al. The simian foamy virus type 1 transcriptional transactivator (Tas) binds and activates an enhancer element in the gag gene. J. Virol. 70: 6847-6855, 1996. PubMed: 8794326 Gonzalez Armas JC, et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed: 8892915 Jang SI, et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Dittrich E, et al. A di-leucine motif and an upstream serine in the interleukin-6 (IL-6) signal transducer gp130 mediate ligand-induced endocytosis and down-regulation of the IL-6 receptor. J. Biol. Chem. 271: 5487-5494, 1996. PubMed: 8621406 Lee JH, et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Chen Y, et al. Demonstration of binding of dengue virus envelope protein to target cells. J. Virol. 70: 8765-8772, 1996. PubMed:8971005 Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528 Wright DA, et al. Association of human fas (CD95) with a ubiquitin-conjugating enzyme (UBC-FAP). J. Biol. Chem. 49: 31037-31043, 1996. PubMed: 8940097 Zhang J, et al. Dynamin and beta-arrestin reveal distinct mechanisms for G protein-coupled receptor internalization. J. Biol. Chem. 271: 18302-18305, 1996. PubMed: 8702465 Ozcelebi F, et al. Phosphorylation of cholecystokinin receptors expressed on chinese hamster ovary cells. J. Biol. Chem. 271: 3750-3755, 1996. PubMed: 8631990 Gibson S, et al. Functional LCK is required for optimal CD28-mediated activation of the TEC family tyrosine kinase EMT/ITK. J. Biol. Chem. 271: 7079-7083, 1996. PubMed: 8636141 Shaul PW, et al. Acylation targets endothelial nitric-oxide synthase to plasmalemmal caveolae. J. Biol. Chem. 271: 6518-6522, 1996. PubMed: 8626455 Ladner RD, et al. Identification of a consensus cyclin-dependent kinase phosphorylation site unique to the nuclear form of human deoxyuridine triphosphate nucleotidohydrolase. J. Biol. Chem. 271: 7752-7757, 1996. PubMed: 8631817 Wu X, et al. Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins. J. Biol. Chem. 271: 10277-10281, 1996. PubMed: 8626595 Hawes BE, et al. Phosphatidylinositol 3-kinase is an early intermediate in the G beta gamma-mediated mitogen-activated protein kinase signaling pathway. J. Biol. Chem. 271: 12133-12136, 1996. PubMed: 8647803 Arai H, Charo IF. Differential regulation of G-protein-mediated signaling by chemokine receptors. J. Biol. Chem. 271: 21814-21819, 1996. PubMed: 8702980 Hsieh CM, et al. APEG-1, a novel gene preferentially expressed in aortic smooth muscle cells, is down-regulated by vascular injury. J. Biol. Chem. 271: 17354-17359, 1996. PubMed: 8663449 Holtmann MH, et al. Multiple extracellular loop domains contribute critical determinants for agonist binding and activation of the secretin receptor. J. Biol. Chem. 271: 14944-14949, 1996. PubMed: 8663161
